Reduction of False Positives in Prokaryotic mRNA Differential Display

Reduction of false positives in prokaryotic mRNA differential display.

inverse PCR primers, P1 and P2, can be used to delete the same internal region from a series of DNA constructs containing the target region. Similarly, an internal deletion can be made by a PCR-ligation-PCR approach as described by Ali and Steinkasserer (1). According to this method, for example, the first-round PCR can be performed in two seperate reactions by primers V1/P2 and P1/V2, followed...

Minimizing false positives in differential display.

Fusion proteins could generate false positives in peptide phage display.

Fusion proteins are frequently used in the functional characterization of newly discovered proteins and to identify interacting partners. In our study of hPTP1E, a cytosolic protein tyrosine phosphatase, we used glutathione S-transferase (GST)-fusion protein of the second PDZ domain to identify interacting peptide motifs by peptide phage display. A consensus motif G X X V W L G was identified a...

Elimination of false positives generated through PCR re-amplification of differential display cDNA.

Differential display (DD) is a powerful molecular tool that allows the identification and subsequent isolation of transcripts differentially expressed between biological samples, for example, between undifferentiated and differentiated cells, between different tissues or in one tissue at different stages of development. However, significantly high rates of apparent false positives have been rep...

Reduction of False Positives using Optimal Node Selection in MANET

Mobile Ad-hoc Network (MANET) is a self configuring network which allows nodes to enter the network and also leave the network in a random manner without notification to the network. This dynamic nature allows the malicious nodes to enter the network and attack other legitimate node. To ensure the security in network it is necessary to detect attacks or malicious nodes and notify the network. I...